ZENON Special Alkaline Electrolyzed Water/SDGs Report (4) Joint research with Kagoshima University and Campus Create Co. ~Research theme: Efficacy in disinfection and cleaning~.

2024.04.17 13:00

株式会社ゼノン

Zenon Corporation (Headquarters: Nakano-ku, Tokyo; President: Akinori Abe) has received a license to use the MBT logo mark for ZENON special alkaline electrolyzed water generated by its proprietary technology from the Nara Medical University-MBT Consortium as a product that is medically correct and is expected to make a significant contribution to society. The fourth installment of the "SDG Report," which was started as "SDG Report (4)," was released on our official website on April 8, 2024.

The fourth report is a joint research project with Kagoshima University and Campus Create Co., Ltd. under the following three research themes, and uses three test reports on sterilization and cleaning that have been conducted since April 2022.

<Details: https://znn.co.jp/news/ >

(1) Sterilization: Verification of inhibitory effect on E. coli floating cell proliferation and biofilm formation

(2) Cleaning: Verification of the cleaning effect on oil and grease, which adhere strongly to the walls of fermentation tanks during the brewing process of shochu (distilled spirit) and are difficult to clean.

(3) Comparison】Comparative verification of antibacterial and cleaning effects of alcohol and chemical detergents using the kitchen of a restaurant (Kagoshima City).

*Researchers: Department of Food Creation Science, Graduate School of Agriculture, Forestry and Fisheries, Kagoshima University

　　　　　Makiko Ogata, Toukyou Wu, Yoshinori Kamiya (Associate Professor)

*Coordinator: Campus Create Co.

In the report, ZENON special alkaline electrolyzed water is described as "SAW = Super Alkaline Electrolyzed Water" and strong alkaline electrolyzed water made by other companies is compared as "AlEW = Strong Alkaline Electrolyzed Water.

Kagoshima University

Verification of washing effect on potato stains

Comparison of washing and sterilizing effects in restaurants

Results of ATP wiping test

[Research report / simplified version

*The full text is available on our website

https://znn.co.jp/news/

Introduction

This study examines the sterilizing and cleaning effects and efficacy of super alkaline electrolyzed water as advocated on the Web and other media, as a joint research project of Zeno Co.

Properties of super alkaline electrolyzed water

SAW (=ZENON special alkaline electrolyzed water) may have a bactericidal effect by inactivating coronaviruses and inhibiting the growth of E. coli, etc., due to its strong basicity, and it has been confirmed through joint commissioned experiments by Zenon Corporation (Nara Medical University) that it has a washing effect for grease and oil, etc. SAW is expected to be a new cleaning agent with both cleaning and sterilizing effects. SAW is expected to be a new cleaning agent that combines both cleaning and sterilizing effects.

(1) Sterilization

(1) Verification of inhibitory effect on E. coli floating cell proliferation and biofilm formation

Summary/Results

Biofilms adhering to food contact surfaces can cause cross-contamination and result in outbreaks of foodborne illnesses with serious threats to food safety. These effects of SAW are promising as an alternative to drugs when used as a new cleaning agent in restaurants to maintain a hygienic environment.

(1) Antimicrobial activity of super alkaline electrolyzed water (SAW)

The number of surviving E. coli bacteria after SAW treatment showed a decreasing trend, confirming that SAW has an antimicrobial effect against E. coli bacteria. 《Omission...》

(2) Antimicrobial effect on E. coli floating cells on food contact surfaces

Figure 2 shows the results of the antibacterial effect of each sample water on E. coli floating cells on these two food contact surfaces in this experiment. 《Subsequent omissions》

(3) Observation of E. coli biofilm on food contact surfaces

AlEW (strongly alkaline electrolyzed water made by another company) reduced the amount of E. coli biofilm, but could not completely remove it, while SAW was below the detection limit after 8-12 hours, indicating that it had a higher bactericidal effect than AlEW. ¡§See below.

[(2) Cleaning section].

Verification of the cleaning effect of "sweet potato derived oil and fat" adhered to contaminated samples

Results

Results] Washing effect of super alkaline electrolyzed water (SAW) on "sweet potato stain

It was confirmed that the high basicity of SAW helped to remove contaminants from food contact surfaces. In addition, we confirmed that the cleaning effect was equivalent to that of commercial detergents used in shochu breweries. The events confirmed on the stainless steel surface have promising prospects in the food industry, as they were observed on the glass surface as well. 》

[(3) Cleaning section].

Comparative verification of the cleaning and sterilizing effects of super alkaline electrolyzed water and alcohol/chemical detergents in restaurants (local cuisine restaurants)

Purpose/Overview

In order to solve environmental problems caused by domestic wastewater, it is expected that super-alkaline electrolyzed water generated from water and potassium carbonate will be used for cleaning as a substitute for chemical agents. The cleaning and disinfection effects were verified in comparison with those of "alcohol" and "chemical detergents" actually used in restaurants serving local cuisine.

Results/Discussion

Super alkaline electrolyzed water is considered to have an antimicrobial effect equivalent to that of 78% alcohol, depending on the material of the object. As a future prospect, we will verify the washing effect of super alkaline electrolyzed water and pursue the differences in washing and sterilizing effects seen in different types of stains, such as protein stains and lipid stains. <<Figure 2

Figure 1 Results of general viable bacteria count measurement